tslp cat e el h1598  (Elabscience Biotechnology)

Journal: Cell Reports Medicine

Article Title: Clinical-proteomic classification and precision treatment strategy of chordoma

doi: 10.1016/j.xcrm.2024.101757

Figure Lengend Snippet: Imageological-proteomic analysis of chordoma (A) Representative CT manifestation of sclerotic and osteolytic chordoma. Protein levels (B) and Representative IHC images (C) of RETN in chordoma with or without bone destruction. GSEA enrichment analysis of DEPs between chordoma with or without bone destruction revealed statistic difference in degradation of the ECM (D) and osteopontin signaling (E). (F) Representative T1-weighted images of MRI for chordoma (hypointensity, isointensity, heterogeneous hyperintensity). (G) Kaplan-Meier curve of OS between hypo-/iso-intensity and heterogeneous hyperintensity. Protein levels (H) and representative IHC images (I) of AHNAK2 in chordoma hypo-/iso-intensity or heterogeneous hyperintensity. GSEA enrichment analysis of DEPs between hypo-/iso-intensity and heterogeneous hyperintensity revealed statistic difference in mTORC1 signaling (J) and G2M DNA damage checkpoint (K). Abbreviations: CT, computed tomography; IHC, immunohistochemistry; OS, overall survival; DEPs, differentially expressed proteins; GSEA, gene set enrichment analysis; ECM, extracellular matrix; MRI, magnetic resonance imaging.

Article Snippet: RETN Polyclonal antibody , Proteintech , Cat# 18170-1-AP; RRID: AB_2918058.

Techniques: Computed Tomography, Immunohistochemistry, Magnetic Resonance Imaging

Journal: Cell Reports Medicine

Article Title: Clinical-proteomic classification and precision treatment strategy of chordoma

doi: 10.1016/j.xcrm.2024.101757

Figure Lengend Snippet:

Article Snippet: RETN Polyclonal antibody , Proteintech , Cat# 18170-1-AP; RRID: AB_2918058.

Techniques: Diagnostic Assay, Recombinant, Sequencing, Software

Journal: International Journal of Molecular Sciences

Article Title: Intermittent Hypoxia Up-Regulates CCL2 , RETN, and TNFα mRNAs in Adipocytes via Down-regulation of miR-452

doi: 10.3390/ijms20081960

Figure Lengend Snippet: The mRNA levels of Retn , Tnfα, and Ccl2 in 3T3-L1 cells (preadipocyte and adipocyte) and SW872 cells treated by normoxia or IH for 24 h. The levels of the adipokine mRNAs were measured by real-time RT-PCR using Rig/RpS15 (in mouse) and β-actin (in human) as an endogenous control. Data is expressed as mean ± SE for each group ( n = 4). The statistical analyses were performed using Student’s t -test.

Article Snippet: Cells were exposed to either normoxia or IH for 24 h, culture medium was collected, and the concentration of RETN, TNFα, and CCL2 was measured by using a Human Resistin (RETN) ELISA kit (R&D Systems, Minneapolis, MN), Human TNFα ELISA kit (Diaclone SAS, Besançon, France) and Human C-C motif chemokine ligand 2 (CCL2) ELISA kit (R&D Systems) according to the instructions of the suppliers.

Techniques: Quantitative RT-PCR, Control

Journal: International Journal of Molecular Sciences

Article Title: Intermittent Hypoxia Up-Regulates CCL2 , RETN, and TNFα mRNAs in Adipocytes via Down-regulation of miR-452

doi: 10.3390/ijms20081960

Figure Lengend Snippet: The mRNA levels of Lep , Adip , Retn , Ccl2 , Tnfα , and IL-6 in 3T3-L1 cells treated by normoxia or IH for 24 h. Figure shows relative comparison of mRNA expression in 3T3-L1 preadipocytes with 3T3-L1 adipocytes. The levels of the adipokine mRNAs were measured by real-time RT-PCR using Rig/RpS15 as an endogenous control. Data are expressed as mean ± SE for each group ( n = 4). The statistical analyses were performed using Student’s t -test.

Article Snippet: Cells were exposed to either normoxia or IH for 24 h, culture medium was collected, and the concentration of RETN, TNFα, and CCL2 was measured by using a Human Resistin (RETN) ELISA kit (R&D Systems, Minneapolis, MN), Human TNFα ELISA kit (Diaclone SAS, Besançon, France) and Human C-C motif chemokine ligand 2 (CCL2) ELISA kit (R&D Systems) according to the instructions of the suppliers.

Techniques: Comparison, Expressing, Quantitative RT-PCR, Control

Journal: International Journal of Molecular Sciences

Article Title: Intermittent Hypoxia Up-Regulates CCL2 , RETN, and TNFα mRNAs in Adipocytes via Down-regulation of miR-452

doi: 10.3390/ijms20081960

Figure Lengend Snippet: Concentrations of RETN, TNFα, and CCL2 in SW872 cell culture medium were measured by ELISA. SW872 cells were treated by normoxia or IH for 24 h. Data are expressed as means ± SE for each group ( n = 3).

Article Snippet: Cells were exposed to either normoxia or IH for 24 h, culture medium was collected, and the concentration of RETN, TNFα, and CCL2 was measured by using a Human Resistin (RETN) ELISA kit (R&D Systems, Minneapolis, MN), Human TNFα ELISA kit (Diaclone SAS, Besançon, France) and Human C-C motif chemokine ligand 2 (CCL2) ELISA kit (R&D Systems) according to the instructions of the suppliers.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Intermittent Hypoxia Up-Regulates CCL2 , RETN, and TNFα mRNAs in Adipocytes via Down-regulation of miR-452

doi: 10.3390/ijms20081960

Figure Lengend Snippet: Luciferase assays of promoter activities of RETN , TNFα , and CCL2 in SW872 cells. Reporter plasmids prepared by inserting the promoter fragments of RETN (−979~+20), TNFα (−966~+19), and CCL2 (−3455~+25), upstream of a firefly luciferase reporter gene in pGL4.17 vector were transfected into SW872 cells. After cells were exposed either to IH or normoxia for 24 h, the cells were lysed and the promoter activities of RETN , TNFα , and CCL2 were measured. All data are represented as the mean ± SE of the samples ( n = 5–6). The statistical analyses were performed using Student’s t -test.

Article Snippet: Cells were exposed to either normoxia or IH for 24 h, culture medium was collected, and the concentration of RETN, TNFα, and CCL2 was measured by using a Human Resistin (RETN) ELISA kit (R&D Systems, Minneapolis, MN), Human TNFα ELISA kit (Diaclone SAS, Besançon, France) and Human C-C motif chemokine ligand 2 (CCL2) ELISA kit (R&D Systems) according to the instructions of the suppliers.

Techniques: Luciferase, Plasmid Preparation, Transfection

Journal: International Journal of Molecular Sciences

Article Title: Intermittent Hypoxia Up-Regulates CCL2 , RETN, and TNFα mRNAs in Adipocytes via Down-regulation of miR-452

doi: 10.3390/ijms20081960

Figure Lengend Snippet: Effects of miR-452 mimic transfection on RETN , TNFα , and CCL2 expression. The miR-452 mimic (5′-AACUGUUUGCAGAGGAAACUG-3′, 5′-GUUUCCUCUCUGCAAACAGUUUU-3′) and non-specific control RNA (miR-452 mimic NC) (5′-UUCUCCGAACGUGUCACGUtt-3′, 5′-ACGUGACACGUUCGGAGAAtt-3′) were synthesized by Nihon Gene Research Laboratories, Inc. (NGRL; Sendai, Japan) and introduced into SW872 cells using Lipofectamine ® RNAiMAX just before IH/normoxia exposure, and the mRNA levels of RETN , TNFα , and CCL2 were measured by real-time RT-PCR, as described in Materials and Methods. The expression of RETN , TNFα , and CCL2 mRNA was measured by real-time RT-PCR using β-actin as an endogenous control. Figure represents ( A ) RETN mRNA expression in miR-452 mimic NC-introduced cells, ( B ) RETN mRNA expression in miR-452 mimic-introduced cells, ( C ) TNFα mRNA expression in miR-452 mimic NC-introduced cells, ( D ) TNFα mRNA expression in miR-452 mimic-introduced cells, ( E ) CCL2 mRNA expression in miR-452 mimic NC-introduced cells, and ( F ) CCL2 mRNA expression in miR-452 mimic-introduced cells. Data are expressed as mean ± SE for each group ( n = 6). The statistical analyses were performed using Student’s t -test.

Article Snippet: Cells were exposed to either normoxia or IH for 24 h, culture medium was collected, and the concentration of RETN, TNFα, and CCL2 was measured by using a Human Resistin (RETN) ELISA kit (R&D Systems, Minneapolis, MN), Human TNFα ELISA kit (Diaclone SAS, Besançon, France) and Human C-C motif chemokine ligand 2 (CCL2) ELISA kit (R&D Systems) according to the instructions of the suppliers.

Techniques: Transfection, Expressing, Control, Synthesized, Quantitative RT-PCR

Journal: International Journal of Molecular Sciences

Article Title: Intermittent Hypoxia Up-Regulates CCL2 , RETN, and TNFα mRNAs in Adipocytes via Down-regulation of miR-452

doi: 10.3390/ijms20081960

Figure Lengend Snippet: Primers used for real-time RT-PCR.

Article Snippet: Cells were exposed to either normoxia or IH for 24 h, culture medium was collected, and the concentration of RETN, TNFα, and CCL2 was measured by using a Human Resistin (RETN) ELISA kit (R&D Systems, Minneapolis, MN), Human TNFα ELISA kit (Diaclone SAS, Besançon, France) and Human C-C motif chemokine ligand 2 (CCL2) ELISA kit (R&D Systems) according to the instructions of the suppliers.

Techniques: Sequencing